Mind the gap - Relevant design for laboratory oil exposure of fish as informed by a numerical impact assessment model.

Laboratory experiments provide knowledge of species-specific effects thresholds that are used to parameterize impact assessment models of oil contamination on marine ecosystems. Such experiments typically place individuals of species and life stages in tanks with different contaminant concentrations. Exposure concentrations are usually fixed, and the individuals experience a shock treatment being moved from clean water directly into contaminated water and then back to clean water. In this study, we use a coupled numerical model that simulates ocean currents and state, oil dispersal and fate, and early life stages of fish to quantify oil exposure histories, specifically addressing oil spill scenarios of high rates and long durations. By including uptake modelling we also investigate the potential of buffering transient high peaks in exposure. Our simulation results are the basis for a recommendation on the design of laboratory experiments to improve impact assessment model development and parameterization. We recommend an exposure profile with three main phases: i) a gradual increase in concentration, ii) a transient peak that is well above the subsequent level, and iii) a plateau of fixed concentration lasting ∼3 days. In addition, a fourth phase with a slow decrease may be added.

An impact-based environmental risk assessment model toolbox for offshore produced water discharges.

We present a novel approach to environmental risk assessment of produced water discharges based on explicit impact and probability, using a combination of transport, fate and toxicokinetic-toxicodynamic models within a super-individual framework, with a probabilistic element obtained from ensemble simulations. Our approach is motivated by a need for location and species specific tools which also accounts for the dynamic nature of exposure and uptake of produced water components in the sea. Our approach is based on the well-established fate model DREAM, and accounts for time-variable exposure, considers body burden and effects for specific species and stressors, and assesses the probability of impact. Using a produced water discharge in the Barents Sea, with early life stages of spawning haddock, we demonstrate that it is possible to conduct a model-based risk assessment that highlights the effect of natural variations in environmental conditions. The benefits, limitations and potential for further improvements are discussed.

Effects of Exposure Timing on cyp1a Expression, PAH Elimination, and Lipid Utilization in Lumpfish Embryos Exposed to Produced Water.

Intentional discharges of produced water from oil production platforms to the marine environment contain a complex mixture of toxicants, including polycyclic aromatic hydrocarbons (PAHs). Early life stages of fish are highly sensitive to petrogenic exposure, and short-term exposure during critical periods of embryonic development may have detrimental effects on larvae health and survival. However, why different periods are more sensitive to exposure than others are not fully understood. Three identical exposure experiments (48 h, approx. 30 µg/L tPAH, sum 42 PAHs) on lumpfish (Cyclopterus lumpus) embryos were conducted where only exposure timing was varied: 0-48 h post fertilization (hpf, starting before chorion hardening), 36-84 hpf (starting after chorion hardening), and 240-288 hpf (during organogenesis). Total PAH (tPAH) uptake at the end of exposure was 5× higher when exposed during fertilization than when exposed late (during organogenesis). The first evidence of cyp1a induction in lumpfish during embryogenesis was observed after 84 hpf. Early exposure affected lipid droplet coagulation, indicating altered lipid utilization during embryogenesis. Although no significant impacts of exposure were observed on hatching success, hatching was delayed when exposed at the latest time point. This study shows that chorion properties, lipid content, biotransformation potential, and timing of produced water exposure during lumpfish embryogenesis affected PAH uptake and elimination.

An annual profile of the impacts of simulated oil spills on the Northeast Arctic cod and haddock fisheries.

We simulate the combined natural and pollutant-induced survival of early life stages of NEA cod and haddock, and the impact on the adult populations in response to the time of a major oil spill in a single year. Our simulations reveal how dynamic ocean processes, controlling both oil transport and fate and the frequency of interactions of oil with drifting fish eggs and larvae, mediate the magnitude of population losses due to an oil spill. The largest impacts on fish early life stages occurred for spills initiated in Feb-Mar, concomitant with the initial rise in marine productivity and the earliest phase of the spawning season. The reproductive health of the adult fish populations was maintained in all scenarios. The study demonstrates the application of a simulation system that provides managers with information for the planning of development activities and for the protection of fisheries resources from potential impacts.

Impact of flow field resolution on produced water transport in Lagrangian and Eulerian models.

During offshore petroleum production, large volumes of produced water are continuously discharged. The environmental impact from such discharges is typically assessed with numerical models, which simulate the transport and dilution of the produced water plume in order to predict environmental concentrations of its chemical constituents. In this study we investigate the effects of model resolution (800 m and 4 km) on produced water dispersion. We also compare two different types of models, a Lagrangian particle model, and an Eulerian grid-based ocean model to assess the Eulerian consistency of the Lagrangian model. We consider a point source off the coast of mid-Norway, during two different seasons (winter and spring). In general, the two models are in reasonable agreement. We find a substantial difference in tracer distribution and concentrations between the two resolutions, and to a lesser extent between seasons; in particular, the 800 m model shows lower concentrations along the coast.

New insights into submarine tailing disposal for a reduced environmental footprint: Lessons learnt from Norwegian fjords.

Submarine tailing disposal (STD) in fjords from land-based mines is common practice in Norway and takes place in other regions worldwide. We synthesize the results of a multidisciplinary programme on environmental impacts of STDs in Norwegian fjords, providing new knowledge that can be applied to assess and mitigate impact of tailing disposal globally, both for submarine and deep-sea activities. Detailed geological seafloor mapping provided data on natural sedimentation to monitor depositional processes on the seafloor. Modelling and analytical techniques were used to assess the behaviour of tailing particles and process-chemicals in the environment, providing novel tools for monitoring. Toxicity tests showed biological impacts on test species due to particulate and chemical exposure. Hypersedimentation mesocosm and field experiments showed a varying response on the benthos, allowing to determine the transition zone in the STD impact area. Recolonisation studies indicate that full community recovery and normalisation of metal leakage rates may take several decades due to bioturbation and slow burial of sulfidic tailings. The results are synthesised to provide guidelines for the development of best available techniques for STDs.

North sea produced water PAH exposure and uptake in early life stages of Atlantic Cod.

Produced water discharges from offshore oil and gas platforms represent a significant source of petroleum components such as polycyclic aromatic hydrocarbons (PAHs) released to the ocean. High molecular weight PAHs are persistent in the environment and have a potential for bioaccumulation, and the investigation of their fate and uptake pathways in marine life are relevant when assessing environmental risk of produced water discharges. To study the exposure and uptake of 2-5 ring PAHs in early life stages of Atlantic Cod in the North Sea, we run a coupled fate and individual-based numerical model that includes discharges from 26 platforms. We consider 26 different PAH components in produced water which biodegrade with primary degradation rates; intermediate degradation products are not included. Model simulations are run covering multiple years (2009-2012) to study annual exposure variability, while a one-day time slice of spawning products from the peak spawning season are followed. By covering multiple release points and large spatio-temporal scales, we show how individuals can be exposed to produced water from multiple regions in the North Sea. We find that a combination of oceanic fate processes and toxicokinetics lead to markedly different compositions in the predicted internal concentrations of PAHs compared to discharge concentrations; for instance, naphthalene makes up 30% of the total discharged PAHs, but contributes to at most 1% of internal concentrations. In all simulations we find the predicted total internal PAH concentration (26 components) to be below 1.2 nmol/g, a factor of 1000 less than concentrations commonly associated with acute narcotic effects.

Simulating crude oil exposure, uptake and effects in North Atlantic Calanus finmarchicus populations.

A simulation model framework (SYMBIOSES) that includes a 3-dimensional ocean physics and biology model and a model for transport and fate of oil was used to investigate the potential for bioaccumulation and lethal/sublethal effects of oil components in the copepod Calanus finmarchicus in the Lofoten-Vesterålen archipelago of Norway. The oil model is coupled with the biology model by way of a bioaccumulation model, from which mortality and reduction in reproduction are calculated via a total body burden (TBB). The simulation results indicate that copepod body burden levels are affected by the spill type (surface spill, subsea blowout) and the spill timing (spring, autumn). The effects of oil component bioaccumulation on the copepod population for all scenarios are small, though greatest in the subsea blowout scenarios. We attribute this to the limited spatial and temporal overlap between copepods and oil in the environment simulated by the model. The coupling of the processes of oil transport, bioaccumulation/excretion and the associated effects are discussed in the context of the model framework and with a view towards applications for Ecological Risk Assessment (ERA).

On the use of random walk schemes in oil spill modelling.

In oil spill models, vertical mixing due to turbulence is commonly modelled by random walk. If the eddy diffusivity varies with depth, failing to take the derivative of the diffusivity into account in the random walk scheme will lead to incorrect results. Depending on the diffusivity profile, the result may be either over- or underprediction of the amount of surfaced oil. The importance of using consistent random walk schemes has been known for decades in, e.g., the plankton modelling community. However, it appears not to be common knowledge in the oil spill community, with inconsistent random walk schemes appearing even in recent publications. We demonstrate and quantify the error due to inconsistent random walk, using a simplified oil spill model, and two different diffusivity profiles. In the two cases considered, a commonly used inconsistent scheme predicts respectively 54% and 202% the amount of surface oil, compared to a consistent scheme.

Embryonic exposure to produced water can cause cardiac toxicity and deformations in Atlantic cod (Gadus morhua) and haddock (Melanogrammus aeglefinus) larvae.

Regular discharges of produced water from the oil and gas industry represents the largest direct discharge of effluent into the marine environment worldwide. Organic compound classes typically reported in produced water include saturated hydrocarbons, monoaromatic and polyaromatic hydrocarbons (MAHs, PAHs) as well as oxygenated compounds, such as phenols, acids and ketones. This forms a cocktail of known and suspect toxicants, but limited knowledge is yet available on the sub-lethal toxicity of produced water to cold-water marine fish species. In the present work, we conducted a 4-day exposure of embryos of Atlantic cod (Gadus morhua) and haddock (Melanogrammus aeglefinus) to produced water extracts equivalent to 1:50, 1:500 and 1:5000 times dilutions of raw effluent. No significant reduction in survival or hatching success was observed, however, for cod, hatching was initiated earlier for exposed embryos in a concentration-dependent manner. During recovery, significantly reduced embryonic heart rate was observed for both species. After hatch, larvae subjected to embryonic exposure to produced water extracts were smaller, and displayed signs of cardiotoxicity, jaw and craniofacial deformations. In order to improve risk assessment and regulation of produced water discharges, it is important to identify which produced water components contribute to these effects.

Improving oil spill trajectory modelling in the Arctic.

As petroleum development and other activities move further north, the potential for oil spills in ice-covered waters is of great concern. As a tool for contingency planning and forecasting during response, oil spill models play a key role. With the development of new, high-resolution coupled ice-ocean models, better predictions of sea ice are becoming available. We have updated the OSCAR oil spill model to use sea-ice velocity and coverage fields from coupled ice-ocean models to improve simulation of oil fate and transport in ice-covered waters. We describe the implementation of oil transport in the presence of ice, and demonstrate the improvement by considering three case studies. We find clear improvement when taking ice velocity from a coupled ice-ocean model into account, compared to a heuristic model that uses surface current and wind velocity. The difference is found to be especially important in a response situation near the marginal ice zone.

Toxicokinetics of Crude Oil Components in Arctic Copepods.

The risk of accidental oil spills in the Arctic is on the rise due to increased shipping and oil exploration activities, making it essential to calibrate parameters for risk assessment of oil spills to Arctic conditions. The toxicokinetics of crude oil components were assessed by exposing one lipid-poor (CIII) and one lipid-rich (CV) stage of the Arctic copepod Calanus hyperboreus to crude oil WSF (water-soluble fraction). Water concentrations and total body residues (BR), as well as lipid volume fractions, were measured at regular intervals during exposure and recovery. Bioconcentration factors (BCFs) and elimination rates ( ke) for 26 petrogenic oil components were estimated from one-compartment models fitted to the BR data. Our parameters were compared to estimations made by the OMEGA bioaccumulation model, which uses the octanol-water partitioning coefficient ( KOW) in QSAR (quantitative structure-activity relationship) predictions. Our parameters for the lipid-poor CIIIs generally agreed with the OMEGA predictions, while neither the BCFs nor the kes for the lipid-rich CVs fitted within the realistic range of the OMEGA parameters. Both the uptake and elimination rates for the CVs were in general half an order of magnitude lower than the OMEGA predictions, showing an overestimation of these parameters by the OMEGA model.

Assessing impacts of simulated oil spills on the Northeast Arctic cod fishery.

We simulate oil spills of 1500 and 4500m3/day lasting 14, 45, and 90days in the spawning grounds of the commercial fish species, Northeast Arctic cod. Modeling the life history of individual fish eggs and larvae, we predict deviations from the historical pattern of recruitment to the adult population due to toxic oil exposures. Reductions in survival for pelagic stages of cod were 0-10%, up to a maximum of 43%. These reductions resulted in a decrease in adult cod biomass of <3% for most scenarios, up to a maximum of 12%. In all simulations, the adult population remained at full reproductive potential with a sufficient number of juveniles surviving to replenish the population. The diverse age distribution helps protect the adult cod population from reductions in a single year's recruitment after a major oil spill. These results provide insights to assist in managing oil spill impacts on fisheries.

Automatic determination of heart rates from microscopy videos of early life stages of fish.

Toxic effects of organic hydrophobic contaminants include impacts on fish heart rate (HR) and cardiac functioning. Thus, in ecotoxicology as well as aquaculture and even medicine, fish heart functioning plays an important role in application areas. The aim of this study was to assemble a pipeline of image processing and statistical techniques to extract HR information from microscopy videos of the embryo and larval stages of three species of fish (Atlantic cod, haddock, and Atlantic bluefin tuna). The method enables automatic processing for a large number of individuals, saving a significant amount of time compared with manual processing, while simultaneously eliminating the type of errors such a manual process might incur.

Dynamic Links between Lipid Storage, Toxicokinetics and Mortality in a Marine Copepod Exposed to Dimethylnaphthalene.

Efficiently assessing and managing the risks of pollution in the marine environment requires mechanistic models for toxic effects. The General Unified Threshold model for Survival (GUTS) provides a framework for deriving toxicokinetic-toxicodynamic (TKTD) models for the end point survival. Two recurring questions in the application of GUTS concern the most appropriate death mechanism, and whether the total body residue is a proper dose metric for toxic effects. We address these questions with a case study for dimethylnaphthalene in the marine copepod Calanus finmarchicus. A detailed analysis revealed that body residues were best explained by representing copepods with two toxicokinetic compartments: separating structural biomass and lipid storage. Toxicity is most likely related to the concentration in structure, which led to identification of "stochastic death" as the most appropriate death mechanism. Interestingly, the parametrized model predicts that lipid content will have only minor influence on short-term toxicity. However, the toxicants stored in lipids may have more substantial impacts in situations not included in our experiments (e.g., during diapause and gonad maturation), and for contaminant transfer to eggs and copepod predators.

Modeling filtration of dispersed crude oil droplets by the copepod Calanus finmarchicus.

Oil droplets may form and disperse in the water column after an accidental spill of crude oil or petroleum products at sea. Micro-sized oil droplets may be available for filter feeding organisms, such as the copepod Calanus finmarchicus, which has been shown to filter oil droplets. In the present paper, a modeling approach was used to estimate potential ingestion amounts by copepod filtration of oil droplets. The new model was implemented in the OSCAR (Oil Spill Contingency and Response) software suite, and tested for a series of oil spill scenarios and key parameters. Among these, the size of the filtered droplets was found to be the most important factor influencing the model results. Given the assumptions and simplifications of the model, filtration of dispersed crude oil by C. finmarchicus was predicted to affect the fate of 1-40% of the total released oil mass, depending on the release scenario and parameter values used, with the lower end of that range being more probable in an actual spill situation.

Nonsequential two-photon double ionization of atoms: identifying the mechanism.

We develop an approximate model for the process of direct (nonsequential) two-photon double ionization of atoms. Employing the model, we calculate (generalized) total cross sections as well as energy-resolved differential cross sections of helium for photon energies ranging from 39 to 54 eV. A comparison with results of ab initio calculations reveals that the agreement is at a quantitative level. We thus demonstrate that this complex ionization process can be described by the simple model, providing insight into the underlying physical mechanism. Finally, we use the model to calculate generalized cross sections for the two-photon double ionization of neon in the nonsequential regime.